mTOR Pathway Antibody Sampler Kit (HAK21036)
Catalog# HAK21036
Reacts with
-
Human
Western blot analysis of mTOR on different lysates with Rabbit anti-mTOR antibody (ET1608-5) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-5) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: MCF7 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse testis tissue lysate
Lane 9: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 289 kDa
Observed band size: 289 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-5) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.