Mouse Monoclonal Antibody (mAb) Service
HUABIO offers high-quality custom mouse monoclonal antibody services, designed to meet various research needs. We utilize advanced technology platforms, ensuring the success and efficiency of each custom project, from antigen design, immunization, to antibody screening and validation. Our mouse monoclonal antibody service covers the entire process, from mouse immunization to the efficient selection of antibodies that meet specific application requirements, and is widely used in disease research, drug development, diagnostic testing, and other fields.
HIGHLIGHTS
Rapid Workflow – As Fast As 60 Days
From initial immunization to completion of the first subcloning, the entire process takes approximately 60 days — enabling faster project progression and earlier results.
Antigen-Saving Protocol
Only 3–5 mg of protein or peptide antigen is required, making it ideal for precious or hard-to-produce targets while minimizing material costs.
Flexible & Compatible with Classic Protocols
Our rapid mouse hybridoma workflow is fully compatible with traditional methods. You can seamlessly switch between protocols based on project needs — with no compromise on downstream quality or performance.
Mouse Monoclonal Antibody (mAb) Service Details
| Stage | Details | Time | Deliverables | QC Standard |
|---|---|---|---|---|
| Immunogen Preparation | 1. Conventional peptide projects (13-18aa, purity >85%) 2. Modified peptide project A (monophosphorylation/monoacetylation) 3. Modified peptide project B (dual phosphorylation/lactylation/crotonylation/succinylation/methylation) 4. Prokaryotic protein project C (<1000bp gene fragment synthesis + protein expression and purification, purity >85%) 5. Prokaryotic protein project D (customer provides expression plasmid, only protein expression and purification is performed, purity >85%) 6. Eukaryotic protein project E (<1500bp gene fragment synthesis + protein expression and purification, purity >85%) 7. Eukaryotic protein project F (1500-2500bp gene fragment synthesis + protein expression and purification, purity >85%) |
3-8 weeks (depends on immunogen types) | Peptide report, Protein report | Mass Spectrometry Report for peptides, SDS-PAGE result for proteins |
| Mouse Immunization and Serum Titer Test | 5 Balb/c mice for 3 immunizations, 3.5mg immunogens at least (>85% purity, >0.5mg/ml), serum titer standard 1:8100 > 1.0; For more animal immunization: adding one more mouse, and 0.5mg more immunogen are required. |
8-12 weeks | Antiserum report | Indirect ELISA |
| Cell Fusion | One fusion with up to 20 96-well plates | 4-6 weeks | Fusion record and report | Indirect ELISA of undiluted supernatants |
| Subcloning, Expansion, and Cryopreservation | Two rounds of subcloning with up to 10 single clones, 2 vials of each subclonal hybridoma cell line, about 10ml supernatant of each subclonal cell line. For more multi-clones subcloning. | 3 weeks | Indirect ELISA data/Immunogen-positive clones report, 1.5ml antibody supernatant delivery (at least 10 clones) | Cells cannot be provided to clients |
| Clone Picking | According to client's experiment, 2 clones will be picked for antibody production; For more rounds of cell fusion and subcloning to cryopreservation. | / | / | / |
| Hybridoma Sequencing | 2 clones for antibody sequencing. | 2 weeks | (Additional clone picked for production options available) | Indirect ELISA |
| Antibody Production | 1-2mg purified antibody/clone by Protein A/G column. For more antibody production from acistes preparation to purification. | 4 weeks | 1-2mg purified antibody | / |
| Antibody Test | Indirect ELISA assay to check the binding of immunogen and purified antibodies. SDS-PAGE result can be provided if needed. | / | / | / |