Raptor Recombinant Rabbit Monoclonal Antibody [JE59-39]
Catalog# HA722028
Raptor Recombinant Rabbit Monoclonal Antibody [JE59-39]
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
Raptor Recombinant Rabbit Monoclonal Antibody [JE59-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Raptor aa 1,286-1,335 / 1,335.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 149 kDa
Positive Control
293T cell lysate, K-562 cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse brain tissue.
Conjugation
unconjugated
Clone Number
JE59-39
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:500
Target
Function
RPTOR encodes part of a signaling pathway regulating cell growth which responds to nutrient and insulin levels. RPTOR is an evolutionarily conserved protein with multiple roles in the mTOR pathway. The adapter protein and mTOR kinase form a stoichiometric complex. The encoded protein also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. It upregulates S6 kinase, the downstream effector ribosomal protein, and it downregulates the mTOR kinase. RPTOR also has a positive role in maintaining cell size and mTOR protein expression. The association of mTOR and RPTOR is stabilized by nutrient deprivation and other conditions which suppress the mTOR pathway. Multiple transcript variants exist for this gene which encode different isoforms.
Background References
1. Han H et al. N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis. Nat Commun. 2022 Mar
2. Yin Y et al. RPTOR methylation in the peripheral blood and breast cancer in the Chinese population. Genes Genomics. 2022 Apr
Subcellular Location
Lysosome membrane, Cytoplasm, Cytoplasmic granule.
Synonyms
KIAA1303 antibody
KOG1 antibody
Mip1 antibody
P150 target of rapamycin (TOR) scaffold protein antibody
p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody
P150 target of rapamycin (TOR)-scaffold protein antibody
Raptor antibody
Regulatory associated protein of mTOR antibody
Regulatory associated protein of MTOR complex 1 antibody
Regulatory-associated protein of mTOR antibody
ExpandImages
-
Western blot analysis of Raptor on different lysates with Rabbit anti-Raptor antibody (HA722028) at 1/2,000 dilution.
Lane 1: 293T cell lysate (30 µg/Lane)
Lane 2: K-562 cell lysate (30 µg/Lane)
Lane 3: Neuro-2a cell lysate (30 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 149 kDa
Observed band size: 149 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722028) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Raptor antibody (HA722028) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722028) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"