Monoclonal antibodies (mAbs) are created by immunizing a mouse with a target antigen, thereby eliciting an immune response. The B lymphocytes, taken from the immunized mouse spleen, produce antibodies to the antigen. Each B lymphocyte is then fused with an immortal myeloma cell line, allowing the production of unlimited quantities of antibodies.
Due to their recognition of only one epitope, mAbs are best known for their high specificity. Producing highly specific monoclonal antibodies supplies researchers with the benefits of improved target identification and less background signal.
Since a monoclonal antibody is made by cloning a unique while blood cell. All subsequent products developed from this antibody trace back to its original, unique parent cell. This makes for great batch-to-batch reproducibility.
Guaranteed long term supply
Hybridoma serves as a continuous source of monoclonal antibody.