Lysosomal Marker Antibody Sampler Kit (HAK21188)
Catalog# HAK21188
Reacts with
-
Human
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody (HA722827) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: MDA-MB-231 cell lysate
Lane 6: HeLa cell lysate
Lane 7: HeLa cell lysate treated with deglycosylation
Lane 8: NIH/3T3 cell lysate
Lane 9: NIH/3T3 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 120/44 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: A431 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: MDA-MB-231 cell lysate
Lane 6: HeLa cell lysate
Lane 7: HeLa cell lysate treated with deglycosylation
Lane 8: NIH/3T3 cell lysate
Lane 9: NIH/3T3 cell lysate treated with deglycosylation
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 120/44 kDa
Exposure time: 21 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722827) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.