Vimentin Mouse Monoclonal Antibody [D4-B11] (EM0401)
Catalog# EM0401
Mouse Monoclonal to Vimentin
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WB
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IF-Cell
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IHC-P
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FC
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IF-Tissue
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IHC-Fr
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IP
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Human
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Mouse
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Rat
Lane 1: Wild-type Hela whole cell lysate (20 µg).
Lane 2: Vimentin knockout Hela whole cell lysate (20 µg).
EM0401 was shown to specifically react with Vimentin in wild-type Hela cells. No band was observed when Vimentin knockout sample was tested. Wild-type and Vimentin knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0401, 1/500) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Vimentin Mouse Monoclonal Antibody [D4-B11] (EM0401)
Immunogen
Synthetic peptide within c-terminal human vimentin.
Host
Mouse
Positive Control
HeLa cell lysate, C2C12 cell lysate, C6 cell lysate, NIH/3T3 cell lysate, Mouse embryonic stem cell lysate, Hela, human kidney tissue, human colon carcinoma tissue, human stomach carcinoma tissue, human tonsil tissue, human skin tissue, human liver tissue, human appendix tissue, human endometrium tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
D4-B11
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IF-Cell
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1:200
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IHC-P
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1:200
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FC
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1:500-1:1,000
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IF-Tissue
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200-400
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IHC-Fr
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1:100
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IP
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Use at an assay dependent concentration
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 54 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Vimentin
SYNONYMS
CTRCT30 antibody;Epididymis luminal protein 113 antibody;FLJ36605 antibody;HEL113 antibody;VIM antibody;VIME_HUMAN antibody;Vimentin antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
POST-TRANSLATIONAL MODIFICATION
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
Vimentin is a structural protein that in humans is encoded by the VIM gene. Its name comes from the Latin vimentum which refers to an array of flexible rods. Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all animal cells[6] as well as bacteria. Intermediate filaments, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
CITATIONS
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Tao Jing,et al
A nano-platform combats the "attack" and "defense" of cytoskeleton to block cascading tumor metastasis
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WB
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Mouse
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He, X., Ma, Y., Huang, Z., Wang, G., Wang, W., Zhang, R., Guo, G., Zhang, X., Wen, Y., & Zhang, L.
SERPINB5 is a prognostic biomarker and promotes proliferation, metastasis and epithelial-mesenchymal transition (EMT) in lung adenocarcinoma
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WB
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Human
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Citation
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Zhong Xin,et al
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R
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IF
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Mouse
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Citation
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Liu Yang,et al
RGS10 deficiency facilitates distant metastasis by inducing epithelial-mesenchymal transition in breast cancer
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WB
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IHC
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IF
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Mouse
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Citation
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Wang, Z., Li, Y., Zhao, W., Jiang, S., Huang, Y., Hou, J., Zhang, X., Zhai, Z., Yang, C., Wang, J., Zhu, J., Pan, J., Jiang, W., Li, Z., Ye, M., Tan, M., Jiang, H., & Dang, Y.
Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cells
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WB
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Human
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Jin, L., Yu, B., Liu, G., Nie, W., Wang, J., Chen, J., Xiao, L., Xia, H., Han, F., & Yang, Y.
Mitophagy induced by UMI-77 preserves mitochondrial fitness in renal tubular epithelial cells and alleviates renal fibrosis
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WB
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Human
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Citation
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Liu Yangfan,et al
Fractionated irradiation induces radioresistant oral carcinoma cells with enhanced malignant phenotypes
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WB
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Human
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Citation
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He, S., Li, S., Guo, J., Zeng, X., Liang, D., Zhu, Y., Li, Y., Yang, D., & Zhao, X.
CD166-specific CAR-T cells potently target colorectal cancer cells
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WB
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Human
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Citation
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Minglu Zhou,et al
Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition
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IF
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Mouse
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Citation
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Sclareol attenuates liver fibrosis through SENP1‐mediated VEGFR2 SUMOylation and inhibition of downstream STAT3 signaling
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WB
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Rat
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Citation
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Meng, Y., Hu, X., Li, S., Zeng, X., Qiu, L., Wei, M., Wang, Z., & Han, J.
miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1. BMC cancer, 20(1), 1028.
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WB
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Human
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Citation
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TRPM2 protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction via modulating autophagy
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IF-Cell
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Mouse
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Citation
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