PRODUCT CODE: ER2001-55

Zfp352 Rabbit Polyclonal Antibody (ER2001-55)

Applications

  • WB

  • ICC

REACTIVITY

  • Mouse

Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-55, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: Non-transfected 293T cells lysate<br />
Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate<br />
Lane 3: Non-transfected 293F cells lysate<br />
Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate
  • Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-55, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: Non-transfected 293T cells lysate<br />
Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate<br />
Lane 3: Non-transfected 293F cells lysate<br />
Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate
  • ICC staining of Zfp352 in Non-transfected 293T cells and GFP-tagged Zfp352 transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-55, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Zfp352 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-55, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Non-transfected 293T cells lysate
Lane 2: GFP-tagged Zfp352 transfected 293T cells lysate
Lane 3: Non-transfected 293F cells lysate
Lane 4: GFP-tagged Zfp352 transfected 293F cells lysate

Applications

  • WB

  • ICC

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Zfp352 Rabbit Polyclonal Antibody (ER2001-55)

Immunogen

Synthetic peptide within mouse zfp352 aa 100-150.

Host

Rabbit

Positive Control

Non-transfected 293T cells lysate, GFP-tagged Zfp352 transfected 293T cells lysate, Non-transfected 293F cells lysate, GFP-tagged Zfp352 transfected 293F cells lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

62 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Zfp352

SYNONYMS

ZZfp352 antibody; Zinc finger protein 352 antibody

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Zfp352 (formerly 2czf48), that is expressed in early mouse embryos. The Zfp352 gene is intron‐less in the coding region but carries a solitary 4.3‐kb intron in the 5′‐untranslated region.