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Western blot analysis of ZAP70 on human thymus tissue lysates with Rabbit anti-ZAP70 antibody (ET1706-42) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
70 kDa zeta associated protein antibody;70 kDa zeta-associated protein antibody;EC 184.108.40.206 antibody;FLJ17670 antibody;FLJ17679 antibody;Selective T cell defect antibody;SRK antibody;STD antibody;Syk related tyrosine kinase antibody;Syk-related tyrosine kinase antibody;Truncated ZAP kinase antibody;Tyrosine protein kinase ZAP70 antibody;Tyrosine-protein kinase ZAP-70 antibody;TZK antibody;ZAP 70 antibody;ZAP70 antibody;ZAP70_HUMAN antibody;Zeta chain associated protein kinase 70kD antibody;Zeta chain associated protein kinase 70kDa antibody;Zeta chain associated protein kinase 70kDa isoform 1 antibody;Zeta chain associated protein kinase 70kDa isoform 2 antibody;Zeta chain of T cell receptor associated protein kinase 70 antibody;Zeta chain TCR associated protein kinase 70kD antibody;Zeta chain TCR associated protein kinase 70kDa antibody
Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22.; Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
Cytoplasm. Cell membrane.
The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti α and β chains, the CD3 γ, δ and e chains and a ζ-containing homodimer or heterodimer. The disulfide-linked Ti α-β heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti α and β are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and ζ subunits suggest a role in signal transduction. Of these, the ζ chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation.
Yu, J., Gao, W., Gao, S., Wen, S., Zhao, Y., Shang, Z., Wang, Y., & Niu, Y.
Identification of LAT/ZAP70 characterized immune subtypes of prostate cancer