YY1 Recombinant Rabbit Monoclonal Antibody [SY29-01] (ET1605-40)
Catalog# ET1605-40
Rabbit Monoclonal to YY1
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WB
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IHC-P
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IP
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: C2C12 cell lysate
Lane 3: F9 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 68 kDa
Exposure time: 1 minute 21 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-40) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
YY1 Recombinant Rabbit Monoclonal Antibody [SY29-01] (ET1605-40)
Immunogen
Synthetic peptide within human yy1 aa 255-304 / 414.
Host
Rabbit
Positive Control
HeLa cell lysate, C2C12 cell lysate, F9 cell lysate, PC-12 cell lysate, C6 cell lysate, human colon tissue, mouse colon tissue, mouse kidney tissue, rat colon tissue, rat kidney tissue, HeLa, C2C12, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY29-01
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:50-1:200
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
YY1
SYNONYMS
CF1 antibody;Delta antibody;Delta transcription factor antibody;INO80 complex subunit S antibody;INO80S antibody;NF E1 antibody;NF-E1 antibody;NFE1 antibody;OTTHUMP00000197459 antibody;Transcriptional repressor protein YY1 antibody;TYY1_HUMAN antibody;UCR motif DNA binding protein antibody;UCRBP antibody;Yin and yang 1 antibody;Yin and Yang 1 protein antibody;Yin Yang 1 antibody;Ying Yang 1 antibody;YY 1 antibody;YY 1 transcription factor antibody;YY-1 antibody;YY1 antibody;YY1 transcription factor antibody
SEQUENCE SIMILARITIES
Belongs to the YY transcription factor family.
POST-TRANSLATIONAL MODIFICATION
Transiently poly-ADP-ribosylated by PARP1 upon DNA damage, with the effect of decreasing affinity of YY1 to its cognate DNA binding sites.; Ubiquitinated.
SUBCELLULAR LOCATION
Nucleus matrix.
FUNCTION
The YY1 transcription factor, also known as NF-E1 (human) and Delta or UCRBP (mouse) is of interest due to its diverse effects on a wide variety of target genes. YY1 is broadly expressed in a wide range of cell types and contains four C-terminal zinc finger motifs of the Cys-Cys-His-His type and an unusual set of structural motifs at its N-terminal. It binds to downstream elements in several vertebrate ribosomal protein genes, where it apparently acts positively to stimulate transcription and can act either negatively or positively in the context of the immunoglobulin k 3' enhancer and immunoglobulin heavy-chain μE1 site as well as the P5 promoter of the adeno-associated virus. It thus appears that YY1 is a bifunctional protein, capable of functioning as an activator in some transcriptional control elements and a repressor in others.
CITATIONS
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Luo, C., Chen, X., Bai, Y., Xu, L., Wang, S., Yao, L., Guo, X., Wang, D., & Zhong, X.
Upregulation of Yin-Yang-1 Associates with Proliferation and Glutamine Metabolism in Esophageal Carcinoma
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WB
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IHC
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KD
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Human
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Citation
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Hypoxia promotes the expression of Von Willebrand factor in breast cancer cells by up-regulating the transcription factor YY1 and down-regulating the hsa-miR-424
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ChIP
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Human
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Citation
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Lu, Y., Huang, X., Liang, W., Li, Y., Xing, M., Pan, W., Zhang, Y., Wang, Z., & Song, W.
Regulation of TREM2 expression by transcription factor YY1 and its protective effect against Alzheimer's disease
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WB
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Human
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Citation
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