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PRODUCT CODE: ER1902-50

YARS2 Rabbit Polyclonal Antibody (ER1902-50)

  • Zebrafish

Applications

  • IF

  • IHC-P

REACTIVITY

  • Zebrafish

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IF staining of YARS2 in Zebrafish embryos (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • IF staining of YARS2 in Zebrafish embryos (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded Zebrafish embryos tissue section using anti-YARS2 antibody. Counter stained with hematoxylin.
IF staining of YARS2 in Zebrafish embryos (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • IF

  • IHC-P

REACTIVITY

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

YARS2 Rabbit Polyclonal Antibody (ER1902-50)

Immunogen

Recombinant protein within zebrafish yars2 aa 251-474 / 474.

Host

Rabbit

Positive Control

Zebrafish.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • IF:1:50-1:200

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

F1Q4R5

PROTEIN NAME

YARS2

SYNONYMS

CGI 04 antibody; FLJ13995 antibody; mitochondrial antibody; MLASA2 antibody; mt TyrRS antibody; SYYM_HUMAN antibody; Tyrosine tRNA ligase 2 mitochondrial antibody; Tyrosine tRNA ligase antibody; Tyrosine tRNA ligase mitochondrial antibody; Tyrosine--tRNA ligase antibody; Tyrosyl tRNA synthetase 2 mitochondrial antibody; Tyrosyl tRNA synthetase mitochondrial precursor antibody; Tyrosyl-tRNA synthetase antibody; TyrRS antibody; YARS 2 antibody; YARS2 antibody

SEQUENCE SIMILARITIES

Belongs to the class-I aminoacyl-tRNA synthetase family.

SUBCELLULAR LOCATION

Cytosol. Mitochondrion.

FUNCTION

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS2 are the cause of myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2) [MIM:613561]. MLASA2 is a rare oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Affected individuals manifest sideroblastic anemia, progressive lethargy, muscle weakness, and exercise intolerance associated with persistent lactic acidemia.