xCT / SLC7A11 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A7C6-R] (HA610011)
Catalog# HA610011
Mouse Monoclonal to xCT/SLC7A11
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
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Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of xCT / SLC7A11 on different lysates with Mouse anti-xCT / SLC7A11 antibody (HA610011) at 1/500 dilution.
Lane 1: HT-29 cell lysate
Lane 2: A549 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate
Lane 2: A549 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.