xCT / SLC7A11 Mouse Monoclonal Antibody [A7C5] (HA600097)
Catalog# HA600097
Mouse Monoclonal to xCT/SLC7A11
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WB
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IHC-P
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FC
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Human
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Mouse
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 5: MDA-MB-468 cell lysate (20 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600097) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
xCT / SLC7A11 Mouse Monoclonal Antibody [A7C5] (HA600097)
Immunogen
293 cell line overexpressing slc7a11.
Host
Mouse
Positive Control
HCT 116 cell lysate, A549 cell lysate, HT-29 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, human pancreas tissue, A549, HT-29.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A7C5
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
xCT/SLC7A11
SYNONYMS
Amino acid transport system xc xCT antibody antibody;Amino acid transport system xc- antibody;Calcium channel blocker resistance protein CCBR1 antibody;Calcium channel blocker resistance protein CCBR1 antibody antibody;CCBR1 antibody;Cysteine/glutamate transporter antibody antibody;Cystine/glutamate transporter antibody;OTTHUMP00000164578 antibody;SLC7A11 antibody;Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11 antibody;solute carrier family 7 antibody;Solute carrier family 7 member 11 antibody;Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody;SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN antibody;xCT antibody;XCT_HUMAN antibody
SUBCELLULAR LOCATION
Membrane.
FUNCTION
This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death.
CITATIONS
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Xia Yu,et al
Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling
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WB
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Mouse
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Citation
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Boosting ferroptosis and microtubule inhibition for antitumor therapy via a carrier-free supermolecule nanoreactor
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WB
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Mouse
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Citation
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Xu, F., Zhang, J., Ji, L., Cui, W., Cui, J., Tang, Z., Sun, N., Zhang, G., Guo, M., Liu, B., & Dong, J.
Inhibition of Non-small Cell Lung Cancer by Ferroptosis and Apoptosis Induction through P53 and GSK-3β/Nrf2 Signal Pathways using Qingrehuoxue Formula
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WB
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Mouse
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Citation
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Tian, C., Zhao, J., Xiong, Q., Yu, H., & Du, H.
Secondary iron overload induces chronic pancreatitis and ferroptosis of acinar cells in mice
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WB
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Mouse
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Citation
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