PRODUCT CODE: ET1703-23

XBP1 Recombinant Rabbit Monoclonal Antibody [JM10-62] (ET1703-23)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: HepG2<br />
Lane 2: 293T
  • Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: HepG2<br />
Lane 2: 293T
  • ICC staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining XBP1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining XBP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: 293T

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

XBP1 Recombinant Rabbit Monoclonal Antibody [JM10-62] (ET1703-23)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, 293T, HepG2, SW480, human liver tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-62

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

XBP1

SYNONYMS

Tax responsive element binding protein 5 antibody; Tax-responsive element-binding protein 5 antibody; TREB5 antibody; X box binding protein 1 antibody; X box binding protein 2 antibody; X-box-binding protein 1 antibody; XBP 1 antibody; XBP-1 antibody; XBP1 antibody; XBP1_HUMAN antibody; XBP2 antibody

SEQUENCE SIMILARITIES

Belongs to the bZIP family.

TISSUE SPECIFICITY

Expressed in plasma cells in rheumatoid synovium. Over-expressed in primary breast cancer and metastatic breast cancer cells. Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells.

POST-TRANSLATIONAL MODIFICATION

[Isoform 2]: Acetylated by EP300; acetylation positively regulates the transcriptional activity of XBP1 isoform 2. Isoform 2 is deacetylated by SIRT1; deacetylation negatively regulates the transcriptional activity of XBP1 isoform 2.; [Isoform 1]: Ubiquitinated, leading to proteasome-mediated degradation in response to ER stress.; X-box-binding protein 1, cytoplasmic form and luminal form are produced by intramembrane proteolytic cleavage of ER membrane-anchored isoform 1 triggered by HM13/SPP in a DERL1-RNF139-dependent and VCP/p97-independent manner. X-box-binding protein 1, luminal form is ubiquitinated leading to proteasomal degradation.

SUBCELLULAR LOCATION

Nucleus, Chromosome, Chromosome.

FUNCTION

The X-box binding protein-1 (XBP-1 or hXBP-1), also designated tax-responsive element-binding protein 5 (TREB5) in mouse and human, or hepatocarcinogenesis-related transcription factor (HTF) in rat, belongs to the basic region/leucine zipper (bZIP) family of transcription factors. XBP-1 was first characterized as a protein that binds to the HLA-DRα promoter in B cells. XBP-1 recognizes the cAMP responsive element (CRE) in enhancers of human T cell leukemia virus and major histocompatibility complex class II genes and activates transcription of these genes. It is expressed at high levels in developing bone and its levels are modulated during osteoblast development, suggesting a role in regulation of expression of osteoblast-specific genes. In addition to binding to CRE sequences, XBP-1 has been shown to bind to TPA response elements (TREs).