WSCD2 Mouse Monoclonal Antibody [9-F3] (M1010-2)

Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution.

Applications

WB
ICC
IHC-P

REACTIVITY

Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

WSCD2 Mouse Monoclonal Antibody [9-F3] (M1010-2)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Human stomach tissue lysate, Human liver cancer tissue, human thyroid tissue, human uterus tissue, SH-SY5Y cell.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9-F3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Cafter thawing. Aliquot store at -20Cor -80CAvoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgM

APPLICATION DILUTION

WB
1:500-1:2,000

ICC
1:50-1:200

IHC-P
1:100-1:500

TARGET

UNIPROT #

Q2TBF2

PROTEIN NAME

WSCD2

SYNONYMS

4933413A10Rik antibody; C530024P05Rik antibody; Gm450 antibody; WSC domain containing 2 antibody; WSC domain containing protein 2 antibody; WSC domain-containing protein 2 antibody; WSCD 2 antibody; WSCD2 antibody; WSCD2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the WSCD family.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

WSC domain-containing protein 2 (WSCD2) is a 565 amino acid, single-pass membrane protein that contains two WSC domains. The WSC domain is named after the yeast WSC1-WSC4 (cell wall integrity and stress response components 1-4) proteins, which each contain a WSC domain, and is a putative carbohydrate binding domain that spans about 90 amino acids. Containing eight conserved cysteine residues, which are predicted to form disulfide bridges, WSC domains are often found with other domains, such as LDL-receptor class A, PKD and C-type lectin. Two isoforms of WSCD2 exist as a result of alternative splicing events.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY

The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)

Applications

REACTIVITY

Human

Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat