PRODUCT CODE: M1010-2

WSCD2 Mouse Monoclonal Antibody [9-F3] (M1010-2)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution.
  • Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution.
  • Western blot analysis of WSCD2 on human stomach tissue lysate using anti-WSCD2 antibody at 1/1000 dilution.
  • ICC staining WSCD2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-WSCD2 antibody. Counter stained with
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-WSCD2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-WSCD2 antibody. Counter stained with hematoxylin.
Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

WSCD2 Mouse Monoclonal Antibody [9-F3] (M1010-2)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Human stomach tissue lysate, Human liver cancer tissue, human thyroid tissue, human uterus tissue, SH-SY5Y cell.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9-F3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Cafter thawing. Aliquot store at -20Cor -80CAvoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

WSCD2

SYNONYMS

4933413A10Rik antibody; C530024P05Rik antibody; Gm450 antibody; WSC domain containing 2 antibody; WSC domain containing protein 2 antibody; WSC domain-containing protein 2 antibody; WSCD 2 antibody; WSCD2 antibody; WSCD2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the WSCD family.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

WSC domain-containing protein 2 (WSCD2) is a 565 amino acid, single-pass membrane protein that contains two WSC domains. The WSC domain is named after the yeast WSC1-WSC4 (cell wall integrity and stress response components 1-4) proteins, which each contain a WSC domain, and is a putative carbohydrate binding domain that spans about 90 amino acids. Containing eight conserved cysteine residues, which are predicted to form disulfide bridges, WSC domains are often found with other domains, such as LDL-receptor class A, PKD and C-type lectin. Two isoforms of WSCD2 exist as a result of alternative splicing events.