PRODUCT CODE: ET7110-34

WNT5B Recombinant Rabbit Monoclonal Antibody [JE53-67] (ET7110-34)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of WNT5B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: HepG2 cell lysate
  • Western blot analysis of WNT5B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: HepG2 cell lysate
  • Flow cytometric analysis of WNT5B was done on LOVO cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of WNT5B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

WNT5B Recombinant Rabbit Monoclonal Antibody [JE53-67] (ET7110-34)

Immunogen

Synthetic peptide within c terminal human wnt5b.

Host

Rabbit

Positive Control

Hela cell lysate, HepG2 cell lysate, LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE53-67

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size 40 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

WNT5B

SYNONYMS

MGC2648 antibody; Protein Wnt-5b antibody; Wingless type MMTV integration site family, member 5B antibody; WNT 5B antibody; WNT 5B protein antibody; Wnt family member 5B antibody; Wnt5b antibody; WNT5B protein antibody; WNT5B_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the Wnt family.

POST-TRANSLATIONAL MODIFICATION

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

SUBCELLULAR LOCATION

Extracellular matrix.

FUNCTION

Protein Wnt-5b is a protein that in humans is encoded by the WNT5B gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.