PRODUCT CODE: ER1511-4

WNT2 Rabbit Polyclonal Antibody (ER1511-4)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of WNT2 on different lysates using anti-WNT2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
      Lane 2: Mouse brain tissue<br />
 Lane 3: Jurkat <br />
    Lane 4: Human lung tissue
  • Western blot analysis of WNT2 on different lysates using anti-WNT2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
      Lane 2: Mouse brain tissue<br />
 Lane 3: Jurkat <br />
    Lane 4: Human lung tissue
  • Flow cytometric analysis of Jurkat cells with WNT2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of WNT2 on different lysates using anti-WNT2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: Mouse brain tissue
Lane 3: Jurkat
Lane 4: Human lung tissue

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

WNT2 Rabbit Polyclonal Antibody (ER1511-4)

Immunogen

Synthetic peptide within human wnt2 aa 209-263.

Host

Rabbit

Positive Control

PC-3M, mouse brain tissue, Jurkat, human lung tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

WNT2

SYNONYMS

Int 1 related protein antibody; Int-1-like protein 1 antibody; Int-1-related protein antibody; INT1L 1 antibody; INT1L1 antibody; IRP antibody; IRP protein antibody; ONCOGENE INT1 LIKE 1 antibody; Protein Wnt-2 antibody; Secreted growth factor antibody; Wingless type MMTV integration site family member 2 antibody; WNT 2 antibody; WNT2 antibody; WNT2_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the Wnt family.

TISSUE SPECIFICITY

Expressed in brain in the thalamus, in fetal and adult lung and in placenta.

POST-TRANSLATIONAL MODIFICATION

Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Belongs to the Wnt family. Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.