UNIPROT #

Q9H4A3

PROTEIN NAME

WNK1

SYNONYMS

Erythrocyte 65 kDa protein antibody; HSAN2 antibody; HSN2 antibody; hWNK1 antibody; KDP antibody; KIAA0344 antibody; Kinase deficient protein antibody; MGC163339 antibody; MGC163341 antibody; p65 antibody; PPP1R167 antibody; PRKWNK1 antibody; Prostate derived sterile 20 like kinase antibody; Protein kinase lysine deficient 1 antibody; Protein kinase lysine-deficient 1 antibody; Protein kinase with no lysine 1 antibody; Protein phosphatase 1, regulatory subunit 167 antibody; PSK antibody; Serine/threonine protein kinase WNK1 1 antibody; Serine/threonine protein kinase WNK1 2 antibody; Serine/threonine protein kinase WNK1 antibody; Serine/threonine-protein kinase WNK1 antibody; With no K antibody; WNK lysine deficient protein kinase 1 antibody; WNK lysine deficient protein kinase 1 isoform antibody; WNK1 antibody; WNK1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.

TISSUE SPECIFICITY

Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylation at Ser-382 is inhibited by intracellular calcium.; May be O-glycosylated.; Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.