WNK1 Recombinant Rabbit Monoclonal Antibody [JG36-91] (ET7108-41)
Catalog# ET7108-41
Rabbit Monoclonal to WNK1
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WB
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IHC-P
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Human
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Mouse
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Rat
Lysates/proteins at 10 µg/Lane.
Predicted band size: 225/250/280/300 kDa
Observed band size: 250/300 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-41) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
WNK1 Recombinant Rabbit Monoclonal Antibody [JG36-91] (ET7108-41)
Immunogen
Synthetic peptide within human wnk1 aa 33-82 / 2382.
Host
Rabbit
Positive Control
A549 cell lysates, human skin tissue, human breast cancer tissue, mouse testis tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG36-91
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
225/250/280/300 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
WNK1
SYNONYMS
Erythrocyte 65 kDa protein antibody;HSAN2 antibody;HSN2 antibody;hWNK1 antibody;KDP antibody;KIAA0344 antibody;Kinase deficient protein antibody;MGC163339 antibody;MGC163341 antibody;p65 antibody;PPP1R167 antibody;PRKWNK1 antibody;Prostate derived sterile 20 like kinase antibody;Protein kinase lysine deficient 1 antibody;Protein kinase lysine-deficient 1 antibody;Protein kinase with no lysine 1 antibody;Protein phosphatase 1, regulatory subunit 167 antibody;PSK antibody;Serine/threonine protein kinase WNK1 1 antibody;Serine/threonine protein kinase WNK1 2 antibody;Serine/threonine protein kinase WNK1 antibody;Serine/threonine-protein kinase WNK1 antibody;With no K antibody;WNK lysine deficient protein kinase 1 antibody;WNK lysine deficient protein kinase 1 isoform antibody;WNK1 antibody;WNK1_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
TISSUE SPECIFICITY
Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylation at Ser-382 is inhibited by intracellular calcium.; May be O-glycosylated.; Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
CITATIONS
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Xudong Pan,et al
Lnc_011797 promotes ferroptosis and aggravates white matter lesions
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IHC
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Human
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Citation
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