WLS Mouse Monoclonal Antibody [A7C2] (HA600060)
Catalog# HA600060
Mouse Monoclonal to WLS
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of WLS on different lysates with Mouse anti-WLS antibody (HA600060) at 1/20,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600060) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.