WIPI1 Recombinant Rabbit Monoclonal Antibody [JE56-92] (HA720010)
Catalog# HA720010
Rabbit Monoclonal to WIPI1
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WB
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IHC-P
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Human
Lane 1: JAR cell lysate
Lane 2: SW480 cell lysate
Lane 3: SK-MEL-28 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720010) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
WIPI1 Recombinant Rabbit Monoclonal Antibody [JE56-92] (HA720010)
Immunogen
Recombinant protein within human wipi1 aa 20-120.
Host
Rabbit
Positive Control
JAR cell lysate, SW480 cell lysate, SK-MEL-28 cell lysate, human skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE56-92
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 49 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
WIPI1
SYNONYMS
ATG 18 antibody;ATG18 antibody;Atg18 protein homolog antibody;ATG18A antibody;FLJ10055 antibody;WD repeat domain phosphoinositide interacting 1 antibody;WD repeat domain phosphoinositide interacting protein 1 antibody;WD repeat domain phosphoinositide-interacting protein 1 antibody;WD40 repeat protein interacting with phosphoinositides of 49 kDa antibody;WD40 repeat protein interacting with phosphoInositides of 49kDa antibody;WIPI 1 alpha antibody;WIPI 1 antibody;WIPI 49 antibody;WIPI 49 kDa antibody;WIPI-1 antibody;wipi1 antibody;WIPI1_HUMAN antibody;WIPI49 antibody
SUBCELLULAR LOCATION
Endosome, trans-Golgi network, cytoskeleton, clathrin-coated vesicle, preautophagosomal structure membrane.
FUNCTION
This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants.
CITATIONS
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Xianzhe Wang,et al
Nitazoxanide alleviates experimental pulmonary fibrosis by inhibiting the development of cellular senescence
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IF
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Human
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Citation
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