WIF1 Recombinant Rabbit Monoclonal Antibody [JE41-39] (HA721660)
Catalog# HA721660
Rabbit Monoclonal to WIF1
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of WIF1 on different lysates with Rabbit anti-WIF1 antibody (HA721660) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse heart tissue lysate (40 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Rat lung tissue lysate (40 µg/Lane)
Lane 9: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: SW480 cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: Mouse heart tissue lysate (40 µg/Lane)
Lane 7: Mouse lung tissue lysate (40 µg/Lane)
Lane 8: Rat lung tissue lysate (40 µg/Lane)
Lane 9: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.