WDR77 Recombinant Rabbit Monoclonal Antibody [PSH0-07] (HA721260)
Catalog# HA721260
Rabbit Monoclonal to WDR77
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WB
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IHC-P
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FC
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Human
Lane 1: HepG2 cell lysate
Lane 2: 293 cell lysate
Lane 3: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721260) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
WDR77 Recombinant Rabbit Monoclonal Antibody [PSH0-07] (HA721260)
Immunogen
Recombinant protein within human wdr77 aa 1-342 / 342.
Host
Rabbit
Positive Control
Human prostate tissue, human fallopian tube tissue, mouse brain tissue, HepG2 cell lysate, 293 cell lysate, K562 cell lysate, Hela, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH0-07
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
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FC
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
WDR77
SYNONYMS
2610312E17Rik antibody;Androgen receptor cofactor p44 antibody;C79984 antibody;HKMT1069 antibody;MEP 50 antibody;MEP-50 antibody;MEP50 antibody;MEP50_HUMAN antibody;Methylosome protein 50 antibody;MGC2722 antibody;Nbla10071 antibody;p44 antibody;p44/Mep50 antibody;RGD1310479 antibody;RP11 552M11.3 antibody;WD repeat containing protein 77 antibody;WD repeat domain 77 antibody;WD repeat-containing protein 77 antibody;WDR77 antibody
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
Methylosome protein 50 is a protein that in humans is encoded by the WDR77 gene. Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
CITATIONS
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Xiang Liu,et al
Exploring the molecular mechanisms of comorbidity of myocardial infarction and anxiety disorders by combining multiple data sets with in vivo experimental validation
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WB
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Rat
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Citation
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