PRODUCT CODE: ER1803-18

Vitamin D3 Receptor Rabbit Polyclonal Antibody (ER1803-18)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution.<br />
  Positive control:<br />
  Lane 1: U937<br />
  Lane 2: SK-Br-3
  • Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution.<br />
  Positive control:<br />
  Lane 1: U937<br />
  Lane 2: SK-Br-3
  • ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vitamin D Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Vitamin D Receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution.
Positive control:
Lane 1: U937
Lane 2: SK-Br-3

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Vitamin D3 Receptor Rabbit Polyclonal Antibody (ER1803-18)

Immunogen

Recombinant protein within human vitamin d receptor aa 117-297 / 427.

Host

Rabbit

Positive Control

U937, SK-Br-3, LOVO, MCF-7, PC-3M, rat skin tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vitamin D3 Receptor

SYNONYMS

1 25 dihydroxyvitamin D3 receptor antibody; 1 antibody; 1,25 dihydroxyvitamin D3 receptor antibody; 1,25-@dihydroxyvitamin D3 receptor antibody; 25-dihydroxyvitamin D3 receptor antibody; Member 1 antibody; NR1I1 antibody; Nuclear receptor subfamily 1 group I member 1 antibody; PPP1R163 antibody; Protein phosphatase 1, regulatory subunit 163 antibody; VDR antibody; VDR_HUMAN antibody; Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody; Vitamin D hormone receptor antibody; Vitamin D nuclear receptor variant 1 antibody; Vitamin D receptor antibody; Vitamin D3 receptor antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.