PRODUCT CODE: ER1802-40

Vitamin D Receptor Rabbit Polyclonal Antibody (ER1802-40)

Applications

  • WB

  • FC

REACTIVITY

  • Human

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Western blot analysis of Vitamin D Receptor on PC-3M cell and human small intestine tissue lysate using anti-Vitamin D Receptor antibody at 1/500 dilution.
  • Western blot analysis of Vitamin D Receptor on PC-3M cell and human small intestine tissue lysate using anti-Vitamin D Receptor antibody at 1/500 dilution.
  • Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Vitamin D Receptor on PC-3M cell and human small intestine tissue lysate using anti-Vitamin D Receptor antibody at 1/500 dilution.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Vitamin D Receptor Rabbit Polyclonal Antibody (ER1802-40)

Immunogen

Peptide

Host

Rabbit

Positive Control

PC-3M, Human small intestine tissue lysate, LOVO.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vitamin D Receptor

SYNONYMS

1 25 dihydroxyvitamin D3 receptor antibody; 1 antibody; 1,25 dihydroxyvitamin D3 receptor antibody; 1,[email protected] D3 receptor antibody; 25-dihydroxyvitamin D3 receptor antibody; Member 1 antibody; NR1I1 antibody; Nuclear receptor subfamily 1 group I member 1 antibody; PPP1R163 antibody; Protein phosphatase 1, regulatory subunit 163 antibody; VDR antibody; VDR_HUMAN antibody; Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody; Vitamin D hormone receptor antibody; Vitamin D nuclear receptor variant 1 antibody; Vitamin D receptor antibody; Vitamin D3 receptor antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells.