PRODUCT CODE: ET1703-09

Vitamin D Binding protein Recombinant Rabbit Monoclonal Antibody [JM10-36] (ET1703-09)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.
  • Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.
  • ICC staining DBP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DBP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining DBP in SKOV-3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- DBP antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with DBP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of DBP on human lung lysates using anti- DBP antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Vitamin D Binding protein Recombinant Rabbit Monoclonal Antibody [JM10-36] (ET1703-09)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SKOV-3, Hela, HepG2, human kidney tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-36

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Vitamin D Binding protein

SYNONYMS

DBP antibody; DBP/GC antibody; GC antibody; Gc globulin antibody; Gc-globulin antibody; GRD3 antibody; Group specific component antibody; Group specific component vitamin D binding protein antibody; Group-specific component antibody; hDBP antibody; VDB antibody; VDBG antibody; VDBP antibody; Vitamin D binding alpha globulin antibody; Vitamin D-binding protein antibody; VTDB_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the ALB/AFP/VDB family.

TISSUE SPECIFICITY

Expressed in the liver. Found in plasma, ascites, cerebrospinal fluid and urine.

POST-TRANSLATIONAL MODIFICATION

Allele GC*1S is O-glycosylated at Thr-436. The trisaccharide sugar moiety can be modified by the successive removal of neuraminic acid and galactose leaving an O-mceeN-acetyl-galactosamine. This conversion is thought to produce a macrophage-activating factor (Gc-MAF). Only a minor proportion of plasma GC is O-glycosylated. The potential N-glycosylation site predicted at Asn-288 is thought to be nonglycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Vitamin D-binding protein (DBP) is a multi-functional serum protein that binds to the plasma membranes of numerous cell types and mediates a variety of cellular functions. The locus of the DBP protein (also known as group-specific component protein or GC) is located at human chromosome 4q13.3. DBP functions in organ-specific transportation of vitamin D and its metabolites to the various target organs of the vitamin D endocrine system. In addition, DBP has immunomodulatory properties and is able to bind to the surface of leukocytes. DBP binds to the plasma membrane through a chondroitin sulfate proteoglycan. DBP serves as a co-chemotactic factor for C5a to enhance the chemotactic activity of C5a. DBP can also bind to globular Actin with high affinity and is involved in the clearance of Actin from the blood. DBP plays an important role in osteoclast differentiation. The diverse cellular functions of DBP require its cell surface binding ability to mediate different biological processes.