PRODUCT CODE: M1412-1

Vimentin Mouse Monoclonal Antibody [A6-C1] (M1412-1)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis on A431 cell lysates using anti- Vimentin mouse mAb in different dilution.
  • Western blot analysis on A431 cell lysates using anti- Vimentin mouse mAb in different dilution.
  • ICC staining Vimentin in MCF-7 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis on A431 cell lysates using anti- Vimentin mouse mAb in different dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Vimentin Mouse Monoclonal Antibody [A6-C1] (M1412-1)

Immunogen

Peptide

Host

Mouse

Positive Control

A431, MCF-7

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A6-C1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB 1:2,000-1:5,000

  • ICC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Vimentin

SYNONYMS

CTRCT30 antibody; Epididymis luminal protein 113 antibody; FLJ36605 antibody; HEL113 antibody; VIM antibody; VIME_HUMAN antibody; Vimentin antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

TISSUE SPECIFICITY

Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

POST-TRANSLATIONAL MODIFICATION

Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS.; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

SUBCELLULAR LOCATION

Cytoplasm

FUNCTION

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. In essence, vimentin is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin has been shown to eliminate toxic proteins in JUNQ and IPOD inclusion bodies in asymmetric division of asymmetric division of mammalian cell lines. It has been used as a sarcoma tumor marker to identify mesenchyme. Methylation of the vimentin gene has been established as a biomarker of colon cancer and this is being utilized in the development of fecal tests for colon cancer. High levels of DNA methylation in the promotor region have also been associated with markedly decreased survival in hormone positive breast cancers.

CITATIONS

  • Xiang, Xiaocong et al.

    Tex10 promotes stemness and EMT phenotypes in esophageal squamous cell carcinoma via the Wnt/β-catenin pathway. | Oncology Reports [2019]