VEGFD Recombinant Rabbit Monoclonal Antibody [JE54-75] (ET7110-81)
Catalog# ET7110-81
Rabbit Monoclonal to VEGFD
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
-
Mouse
-
Rat
Lane 1: Caco-2 cell lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Rat heart tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 37 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-81) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
VEGFD Recombinant Rabbit Monoclonal Antibody [JE54-75] (ET7110-81)
Immunogen
Recombinant protein within human vegfd aa 181-302 / 354.
Host
Rabbit
Positive Control
Caco-2 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, HUVEC, rat kidney tissue, human small intestine tissue, mouse testis tissue, mouse heart tissue, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE54-75
RRID
APPLICATION DILUTION
-
WB:1:2,000
-
IF-Cell:1:50-1:100
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 40 kDa.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
VEGFD
SYNONYMS
c-fos induced growth factor antibody;c-fos induced growth factor (vascular endothelial growth factor D) antibody;c-fos induced growth factor antibody;c-fos-induced growth factor antibody;FIGF antibody;Vascular endothelial growth factor D antibody;Vascular endothelial growth factor D precursor antibody;Vascular endothelial growth factor D precursor antibody;VEGF D antibody;VEGF-D antibody;VEGFD antibody;VEGFD_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the PDGF/VEGF growth factor family.
TISSUE SPECIFICITY
Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
POST-TRANSLATIONAL MODIFICATION
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family and is active in angiogenesis, lymphangiogenesis, and endothelial cell growth. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-2 and VEGFR-3 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor C. Read-through transcription has been observed between this locus and the upstream PIR (GeneID 8544) locus. Diseases associated with VEGFD include Anal Canal Squamous Cell Carcinoma and Kidney Angiomyolipoma. Among its related pathways are ERK Signaling and Ras signaling pathway. An important paralog of this gene is VEGFC.
