VEGFC Rabbit Polyclonal Antibody (R1307-4)
Catalog# R1307-4
Rabbit Polyclonal to VEGFC
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WB
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Human
Lane 1: HepG2 cell lysate
Lane 2: 293 cell lysate
Lane 3: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47/100 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1307-4) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
VEGFC Rabbit Polyclonal Antibody (R1307-4)
Immunogen
Synthetic peptide within human vegfc aa 246-290.
Host
Rabbit
Positive Control
HepG2 cell lysate, 293 cell lysate, MCF-7 cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
VEGFC
SYNONYMS
Flt 4L antibody;Flt4 ligand antibody;FLT4 ligand DHM antibody;Flt4-L antibody;Flt4L antibody;Vascular endothelial growth factor C antibody;Vascular endothelial growth factor related protein antibody;Vascular endothelial growth factor-related protein antibody;VEGF C antibody;VEGF-C antibody;Vegfc antibody;VEGFC_HUMAN antibody;VRP antibody
SEQUENCE SIMILARITIES
Belongs to the PDGF/VEGF growth factor family.
TISSUE SPECIFICITY
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
POST-TRANSLATIONAL MODIFICATION
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing a heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
SUBCELLULAR LOCATION
Secreted
FUNCTION
VEGF-A plays a paramount role in tumor angiogenesis, VEGF-C has been characterized as an essential lymphangiogenic factor that promotes cancer metastasis. VEGF-C is a ligand for both VEGF-R3 and VEGF-R2 but has a high affinity for VEGF-R3. It has been reported that the VEGF-C/VEGF-R3 axis is activated in subsets of leukemia patients.
CITATIONS
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Cheng Lin,et al
Hemodynamic force dictates endothelial angiogenesis through MIEN1-ERK/MAPK-signaling axis
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WB
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Human
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Citation
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