PRODUCT CODE: ET1701-93

VE Cadherin Recombinant Rabbit Monoclonal Antibody [JJ0941] (ET1701-93)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Mouse

Western blot analysis of VE Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse heart tissue lysate<br />
Lane 2: Mouse placenta tissue lysate<br />
Lane 3: Mouse spleen tissue lysate<br />
Lane 4: Mouse lung tissue lysate
  • Western blot analysis of VE Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse heart tissue lysate<br />
Lane 2: Mouse placenta tissue lysate<br />
Lane 3: Mouse spleen tissue lysate<br />
Lane 4: Mouse lung tissue lysate
Western blot analysis of VE Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse heart tissue lysate
Lane 2: Mouse placenta tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Mouse lung tissue lysate

Applications

  • WB

  • IP

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

VE Cadherin Recombinant Rabbit Monoclonal Antibody [JJ0941] (ET1701-93)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse heart tissue, mouse placenta tissue, mouse spleen tissue, mouse lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0941

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

88/125 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

VE Cadherin

SYNONYMS

7B 4 antibody; 7B4 antibody; 7B4 antigen antibody; CADH5_HUMAN antibody; Cadherin 5 antibody; Cadherin 5 type 2 antibody; Cadherin 5, type 2 (vascular endothelium) antibody; Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody; cadherin, vascular endothelial antibody; cadherin, vascular endothelial, 1 antibody; Cadherin-5 antibody; Cadherin5 antibody; CD 144 antibody; CD144 antibody; CD144 antigen antibody; CDH 5 antibody; CDH5 antibody; CDH5 protein antibody; Endothelial specific cadherin antibody; FLJ17376 antibody; OTTHUMP00000174777 antibody; Vascular endothelial cadherin antibody; Vascular epithelium cadherin antibody; VE Cad antibody; VE-cadherin antibody; VEC antibody

TISSUE SPECIFICITY

Expressed in postnatal endothelial cells of the retinal vascular plexus (at protein level).

DEVELOPMENTAL STAGE

Expressed in endothelial cells of allantois/umbilical vessels at 8.5 dpc (at protein level). During hemangioblast differentiation, expressed in hemogenic endothelium cells and down-regulated in nascent blood precursors.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.; O-glycosylated.

SUBCELLULAR LOCATION

Cell junction, Cell membrane.

FUNCTION

The cadherins are a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including β-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.