Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
This product has been cited in peer reviewed publications, see list HERE
Western blot analysis of VDAC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate
Lane 2: SW480 cell lysate
N2441 antibody;OMP2 antibody;POR1 antibody;hVDAC1 antibody;MGC111064 antibody;Mitochondrial Porin antibody;Outer mitochondrial membrane protein porin 1 antibody;Plasmalemmal porin antibody;Porin 31HL antibody;Porin 31HM antibody;VDAC antibody;VDAC-1 antibody;Vdac1 antibody;VDAC1_HUMAN antibody;Voltage dependent anion channel 1 antibody;Voltage dependent anion selective channel protein 1 antibody;Voltage-dependent anion-selective channel protein 1 antibody;YNL055C antibody;YNL2441C antibody
Belongs to the eukaryotic mitochondrial porin family.
Heart, liver and skeletal muscle.
Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.; Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.
Voltage-dependent anion-selective channel 1 (VDAC-1) is a beta barrel protein that in humans is encoded by the VDAC1 gene located on chromosome 5. It forms an ion channel in the outer mitochondrial membrane (OMM) and also the outer cell membrane. In the OMM, it allows ATP to diffuse out of the mitochondria into the cytoplasm. In the cell membrane, it is involved in volume regulation. Within all eukaryotic cells, mitochondria are responsible for synthesis of ATP among other metabolite needed for cell survival. VDAC1 therefore allows for communication between the mitochondrion and the cell mediating the balance between cell metabolism and cell death. Besides metabolic permeation, VDAC1 also acts as a scaffold for proteins such as hexokinase that can in turn regulate metabolism.