PRODUCT CODE: ET1702-10

VAMP8 Recombinant Rabbit Monoclonal Antibody [JF0963] (ET1702-10)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela       <br />
Lane 2: Mouse kidney
  • Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela       <br />
Lane 2: Mouse kidney
  • Western blot analysis of VAMP8 on U93 cell lysates using anti-VAMP8 antibody at 1/500 dilution.
  • ICC staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with VAMP8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse kidney

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

VAMP8 Recombinant Rabbit Monoclonal Antibody [JF0963] (ET1702-10)

Immunogen

Synthetic peptide wiithin human vamp8 aa1-29 / 100.

Host

Rabbit

Positive Control

SW480, 293T, Hela, mouse brain tissue, mouse kidney tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0963

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

11 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

VAMP8

SYNONYMS

EDB antibody; Endobrevin antibody; VAMP 8 antibody; VAMP-8 antibody; VAMP8 antibody; VAMP8_HUMAN antibody; Vesicle associated membrane protein 8 antibody; Vesicle-associated membrane protein 8 antibody

SEQUENCE SIMILARITIES

Belongs to the synaptobrevin family.

TISSUE SPECIFICITY

Platelets.

SUBCELLULAR LOCATION

Lysosome membrane, Early endosome membrane, Late endosome membrane, Cell membrane.

FUNCTION

Syntaxins were originally thought to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimide-sensitive factor), SNAPs (soluble NSF attachment proteins) and Synaptotagmin. Endobrevin, also designated VAMP-8 or ED, is a 100 amino acid single-pass type IV membrane protein that belongs to the synaptobrevin family. Similar in sequence to the synaptobrevins, endobrevin is abundantly expressed in kidney, moderately expressed in heart and spleen, and slightly expressed in brain, thymus and liver. Endobrevin interacts specifically with the SNAPs, most likely through an endobrevin-containing SNARE complex.