PRODUCT CODE: ET7109-03

VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19] (ET7109-03)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse cerebellum<br />
  Lane 2: Rat brain
  • Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse cerebellum<br />
  Lane 2: Rat brain
  • ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
Western blot analysis of VAMP1 on different tissue lysates using anti-VAMP1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: Mouse cerebellum
Lane 2: Rat brain

Applications

  • WB

  • IHC-P

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

VAMP1 Recombinant Rabbit Monoclonal Antibody [JG40-19] (ET7109-03)

Immunogen

Full length recombinant protein of human vamp1.

Host

Rabbit

Positive Control

Mouse cerebellum tissue, rat brain tissue, 293T, Hela, SH-SY-5Y, human prostate cancer tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG40-19

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

13 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

VAMP1

SYNONYMS

DKFZp686H12131 antibody; SYB 1 antibody; SYB1 antibody; Synaptobrevin 1 antibody; Synaptobrevin-1 antibody; Synaptobrevin1 antibody; VAMP 1 antibody; VAMP-1 antibody; Vamp1 antibody; VAMP1_HUMAN antibody; Vesicle associated membrane protein 1 antibody; Vesicle associated membrane protein 1 synaptobrevin 1 antibody; Vesicle-associated membrane protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the synaptobrevin family.

TISSUE SPECIFICITY

Nervous system, skeletal muscle and adipose tissue.

POST-TRANSLATIONAL MODIFICATION

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release.; (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.

SUBCELLULAR LOCATION

Mitochondrion. Synapse. Synaptosome.

FUNCTION

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined.