V5 tag Mouse Monoclonal Antibody [A10-D0-A1] (M1008-2)
Catalog# M1008-2
Mouse Monoclonal to V5 tag
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WB
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ELISA
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IF-Cell
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Species independent
Lane 1: 293T cell lysate
Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate
Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-2) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
V5 tag Mouse Monoclonal Antibody [A10-D0-A1] (M1008-2)
Immunogen
Peptide gkpipnpllgldst (v5 epitope) conjugated with klh.
Host
Mouse
Positive Control
V5-tag recombinant protein.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A10-D0-A1
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:10,000
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ELISA
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1:20,000-1:50,000
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IF-Cell
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Isotype
IgG1
TARGET
PROTEIN NAME
V5 tag
SYNONYMS
GKPIPNPLLGLDST epitope tag antibody;GKPIPNPLLGLDST tag antibody;Protein Rev antibody;Regulator of expression of viral proteins antibody;rev antibody;V5 epitope tag antibody
FUNCTION
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The anti-V5 antibody detects recombinant proteins containing the 14 amino acid GKPIPNPLLGLDST sequence.
CITATIONS
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Zhao, J., Xu, J., Yang, T., Yu, X., Cheng, C., Zhang, T., Ren, Z., Li, N., Yang, F., & Li, G.
Expression, purification and characterisation of a human anti-CDK4 single-chain variable fragment antibody
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WB
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Escherichia coli
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Citation
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c-Cbl-mediated ubiquitination of IRF3 negatively regulates IFN-β production and cellular antiviral response
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WB
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Human
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Citation
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王迪
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages
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WB
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None
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Citation
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Guiying Li
Effects of metal ions on the structure and activity of a human anti-cyclin D1 single-chain variable fragment AD5
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WB
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None
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Citation
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