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PRODUCT CODE: ER1706-23

USP36 Rabbit Polyclonal Antibody (ER1706-23)

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

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ICC staining USP36 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP36 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP36 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP36 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP36 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-USP36 antibody. Counter stained with hematoxylin.
ICC staining USP36 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP36 Rabbit Polyclonal Antibody (ER1706-23)

Immunogen

Synthetic peptide within human usp36 aa 1,074-1,123 / 1,123.

Host

Rabbit

Positive Control

A549, Hela, LOVO, human liver cancer tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

123 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q9P275

PROTEIN NAME

USP36

SYNONYMS

Deubiquitinating enzyme 1 antibody; Deubiquitinating enzyme 36 antibody; DUB1 antibody; FLJ12851 antibody; KIAA1453 antibody; Ubiquitin carboxyl-terminal hydrolase 36 antibody; Ubiquitin specific peptidase 36 antibody; Ubiquitin specific processing protease 36 antibody; Ubiquitin thioesterase 36 antibody; Ubiquitin thiolesterase 36 antibody; Ubiquitin-specific-processing protease 36 antibody; UBP36_HUMAN antibody; Usp36 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family.

TISSUE SPECIFICITY

Broadly expressed.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated by NEDD4L, no effect on USP36 protein levels. Both proteins interact with and regulate each other's ubiquitination levels.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Ubiquitin-specific protease 36 is an enzyme that in humans is encoded by the USP36 gene. This gene encodes a member of the ubiquitin-specific protease family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the nucleoli and deubuquitinate nucleophosmin and fibrillarin. May be required for maintaining multiple types of adult stem cells. May function as a transcriptional repressor by continually deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, thereby preventing histone H3 'Lys-4' trimethylation (H3K4).