PRODUCT CODE: EM1901-46

USP28 Mouse Monoclonal Antibody [A1F9] (EM1901-46)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis of USP28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: K562 cell lysate<br />
Lane 2: A549 cell lysate
  • Western blot analysis of USP28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: K562 cell lysate<br />
Lane 2: A549 cell lysate
Western blot analysis of USP28 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

USP28 Mouse Monoclonal Antibody [A1F9] (EM1901-46)

Immunogen

Recombinant protein within human usp28 aa 509-860 / 1,077.

Host

Mouse

Positive Control

K562 cell lysates, A549 cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A1F9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

122 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB:1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

USP28

SYNONYMS

Deubiquitinating enzyme 28 antibody; KIAA1515 antibody; Ubiquitin carboxyl terminal hydrolase 28 antibody; Ubiquitin carboxyl-terminal hydrolase 28 antibody; ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody; Ubiquitin specific peptidase 28 antibody; Ubiquitin specific processing protease 28 antibody; Ubiquitin specific protease 28 antibody; Ubiquitin thioesterase 28 antibody; Ubiquitin thiolesterase 28 antibody; Ubiquitin-specific-processing protease 28 antibody; UBP28_HUMAN antibody; USP 28 antibody; USP28 antibody; USP28 protein antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. USP28 subfamily.

POST-TRANSLATIONAL MODIFICATION

Degraded upon nickel ion level or hypoxia exposure.; Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1.

SUBCELLULAR LOCATION

Nucleoplasm.

FUNCTION

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.