PRODUCT CODE: ER1901-67

USP21 Rabbit Polyclonal Antibody (ER1901-67)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of USP21 on SHSY5Y cell  lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of USP21 on SHSY5Y cell  lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-67, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-67, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-67, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of USP21 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-67, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
Western blot analysis of USP21 on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP21 Rabbit Polyclonal Antibody (ER1901-67)

Immunogen

Recombinant protein within human usp21 aa 303-453 / 565.

Host

Rabbit

Positive Control

SHSY5Y cell lysates, Rat kidney tissue, human fetal skeletal muscle tissue, Mouse heart tissue, SHSY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • IHC-P

  • 1:50-1

  • 200

  • FC

  • 1:50-1

  • 200

TARGET

UNIPROT #

PROTEIN NAME

USP21

SYNONYMS

Deubiquitinating enzyme 21 antibody; deubiquitinating enzyme 23 antibody; EC 3.1.2.15 antibody; ESTM28 antibody; MGC3394 antibody; NEDD8-specific protease antibody; Ubiquitin carboxyl terminal hydrolase 21 antibody; ubiquitin carboxyl terminal hydrolase 23 antibody; Ubiquitin carboxyl-terminal hydrolase 21 antibody; ubiquitin specific peptidase 21 antibody; Ubiquitin specific processing protease 21 antibody; ubiquitin specific protease 16 antibody; ubiquitin specific protease 21 antibody; ubiquitin specific protease 23 antibody; Ubiquitin thioesterase 21 antibody; Ubiquitin thiolesterase 21 antibody; ubiquitin thiolesterase 23 antibody; ubiquitin-specific protease 23, formerly antibody; Ubiquitin-specific-processing protease 21 antibody; UBP21_HUMAN antibody; USP16 antibodyv Usp21 antibody; USP23 antibody; USP23, formerly antibody; W53272 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. USP21 subfamily.

TISSUE SPECIFICITY

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP21 (ubiquitin specific peptidase 21), also known as USP16 or USP23, is a 565 amino acid protein that belongs to the C19 peptidase family of ubiquitin carboxy-terminal hydrolases. Capable of removing ubiquitin from ubiquitinated proteins, USP21 plays a role in signal transduction and can also remove NEDD8 from NEDD8-conjugated proteins, possibly functioning to influence NEDD8-mediated protein proteolysis. Multiple isoforms of USP21 exist due to alternative splicing events.