PRODUCT CODE: ER1802-25

USP21 Rabbit Polyclonal Antibody (ER1802-25)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

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Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.
  • Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.
  • ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining USP21 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP21 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with USP21 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

USP21 Rabbit Polyclonal Antibody (ER1802-25)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse thymus tissue lysate, PC-3M, SH-SY-5Y, human liver cancer tissue, human kidney tissue, human fetal skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

USP21

SYNONYMS

Deubiquitinating enzyme 21 antibody; deubiquitinating enzyme 23 antibody; EC 3.1.2.15 antibody; ESTM28 antibody; MGC3394 antibody; NEDD8-specific protease antibody; Ubiquitin carboxyl terminal hydrolase 21 antibody; ubiquitin carboxyl terminal hydrolase 23 antibody; Ubiquitin carboxyl-terminal hydrolase 21 antibody; ubiquitin specific peptidase 21 antibody; Ubiquitin specific processing protease 21 antibody; ubiquitin specific protease 16 antibody; ubiquitin specific protease 21 antibody; ubiquitin specific protease 23 antibody; Ubiquitin thioesterase 21 antibody; Ubiquitin thiolesterase 21 antibody; ubiquitin thiolesterase 23 antibody; ubiquitin-specific protease 23, formerly antibody; Ubiquitin-specific-processing protease 21 antibody; UBP21_HUMAN antibody; USP16 antibodyv Usp21 antibody; USP23 antibody; USP23, formerly antibody; W53272 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C19 family. USP21 subfamily.

TISSUE SPECIFICITY

Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP21 (ubiquitin specific peptidase 21), also known as USP16 or USP23, is a 565 amino acid protein that belongs to the C19 peptidase family of ubiquitin carboxy-terminal hydrolases. Capable of removing ubiquitin from ubiquitinated proteins, USP21 plays a role in signal transduction and can also remove NEDD8 from NEDD8-conjugated proteins, possibly functioning to influence NEDD8-mediated protein proteolysis. Multiple isoforms of USP21 exist due to alternative splicing events.