Applications
-
WB
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
USP14 Rabbit Polyclonal Antibody (ER1803-55)
Immunogen
Recombinant protein within human usp14 aa 226-408 / 494.
Host
Rabbit
Positive Control
Daudi, SiHa.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
56 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
USP14
SYNONYMS
Deubiquitinating enzyme 14 antibody; TGT antibody; tRNA guanine transglycosylase 60 kD subunit antibody; tRNA guanine transglycosylase antibody; Ubiquitin carboxyl terminal hydrolase 14 antibody; Ubiquitin carboxyl-terminal hydrolase 14 antibody; Ubiquitin specific peptidase 14 antibody; Ubiquitin specific processing protease 14 antibody; Ubiquitin specific protease 14 antibody; Ubiquitin thiolesterase 14 antibody; Ubiquitin-specific-processing protease 14 antibody; UBP14_HUMAN antibody; USP 14 antibody; USP14 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C19 family. USP14/UBP6 subfamily.
SUBCELLULAR LOCATION
Plasma membrane.
FUNCTION
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions. Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.