Positive control:
Lane 1: 293T
Lane 2: Daudi
Lane 3: Mouse testis
Lane 4: Rat brain
Applications
-
WB
-
ICC
-
FC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
USP13 Rabbit Polyclonal Antibody (ER1803-09)
Immunogen
Recombinant protein within human usp13 aa 570-776 / 863.
Host
Rabbit
Positive Control
293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
97 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC
-
1:50-1:200
-
FC
-
1:50-1:100
-
IHC-P
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
USP13
SYNONYMS
Deubiquitinating enzyme 13 antibody; Isopeptidase T 3 antibody; Isopeptidase T-3 antibody; Isopeptidase T3 antibody; ISOT 3 antibody; ISOT-3 antibody; ISOT3 antibody; Ubiquitin carboxyl terminal hydrolase 13 antibody; Ubiquitin carboxyl-terminal hydrolase 13 antibody; Ubiquitin specific peptidase 13 (isopeptidase T3) antibody; Ubiquitin specific peptidase 13 antibody; Ubiquitin specific processing protease 13 antibody; Ubiquitin specific protease 13 antibody; Ubiquitin thioesterase 13 antibody; Ubiquitin thiolesterase 13 antibody; Ubiquitin-specific-processing protease 13 antibody; UBP13 antibody; UBP13_HUMAN antibody; USP 13 antibody; USP13 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C19 family.
TISSUE SPECIFICITY
Highly expressed in ovary and testes.
SUBCELLULAR LOCATION
Nucleus. Cytosol.
FUNCTION
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.