USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38] (ET1706-14)
Catalog# ET1706-14
Rabbit Monoclonal to USP13
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WB
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IP
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Human
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Mouse
Lysates/proteins at 20 µg/Lane.
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-14) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
USP13 Recombinant Rabbit Monoclonal Antibody [JU72-38] (ET1706-14)
Immunogen
Synthetic peptide within human usp13 aa 739-788 / 863.
Host
Rabbit
Positive Control
SH-SY5Y cell lysates, mouse testis tissue lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU72-38
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 97 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
USP13
SYNONYMS
Deubiquitinating enzyme 13 antibody;Isopeptidase T 3 antibody;Isopeptidase T-3 antibody;Isopeptidase T3 antibody;ISOT 3 antibody;ISOT-3 antibody;ISOT3 antibody;Ubiquitin carboxyl terminal hydrolase 13 antibody;Ubiquitin carboxyl-terminal hydrolase 13 antibody;Ubiquitin specific peptidase 13 (isopeptidase T3) antibody;Ubiquitin specific peptidase 13 antibody;Ubiquitin specific processing protease 13 antibody;Ubiquitin specific protease 13 antibody;Ubiquitin thioesterase 13 antibody;Ubiquitin thiolesterase 13 antibody;Ubiquitin-specific-processing protease 13 antibody;UBP13 antibody;UBP13_HUMAN antibody;USP 13 antibody;USP13 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C19 family.
TISSUE SPECIFICITY
Highly expressed in ovary and testes.
SUBCELLULAR LOCATION
Nucleus. Cytoplasm.
FUNCTION
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin.
CITATIONS
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Chloroquine Intervenes Nephrotoxicity of Nilotinib through Deubiquitinase USP13‐Mediated Stabilization of Bcl‐XL
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WB
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Mouse
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Citation
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