PRODUCT CODE: HA720018

Uridine Phosphorylase 1 Recombinant Rabbit Monoclonal Antibody [JE56-62] (HA720018)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate<br />
Lane 2: Mouse kidney tissue lysate<br />
Lane 3: Rat lung tissue lysate<br />
Lane 4: Rat kidney tissue lysate
  • Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate<br />
Lane 2: Mouse kidney tissue lysate<br />
Lane 3: Rat lung tissue lysate<br />
Lane 4: Rat kidney tissue lysate
Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat lung tissue lysate
Lane 4: Rat kidney tissue lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Uridine Phosphorylase 1 Recombinant Rabbit Monoclonal Antibody [JE56-62] (HA720018)

Immunogen

Synthetic peptide within human uridine phosphorylase 1 aa 1-50.

Host

Rabbit

Positive Control

A431 cell lysate, mouse kidney tissue lysate, rat lung tissue lysate, rat kidney tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE56-62

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 34 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Uridine Phosphorylase 1

SYNONYMS

UDRPASE antibody; UP antibody; UPase 1 antibody; UPASE antibody; UPP antibody; Upp1 antibody; UPP1_HUMAN antibody; UrdPase 1 antibody; Uridine phosphorylase 1 antibody

SUBCELLULAR LOCATION

Cytosol, nucleoplasm.

FUNCTION

This gene encodes a uridine phosphorylase, an enzyme that catalyzes the reversible phosphorylation of uridine (or 2'- deoxyuridine) to uracil and ribose-1-phosphate (or deoxyribose-1-phosphate). The encoded enzyme functions in the degradation and salvage of pyrimidine ribonucleosides.