PRODUCT CODE: HA720018

Uridine Phosphorylase 1 Recombinant Rabbit Monoclonal Antibody [JE56-62] (HA720018)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate<br />
Lane 2: Mouse kidney tissue lysate<br />
Lane 3: Rat lung tissue lysate<br />
Lane 4: Rat kidney tissue lysate
  • Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate<br />
Lane 2: Mouse kidney tissue lysate<br />
Lane 3: Rat lung tissue lysate<br />
Lane 4: Rat kidney tissue lysate
Western blot analysis of Uridine Phosphorylase 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA720018, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Rat lung tissue lysate
Lane 4: Rat kidney tissue lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Uridine Phosphorylase 1 Recombinant Rabbit Monoclonal Antibody [JE56-62] (HA720018)

Immunogen

Synthetic peptide within n-terminal human uridine phosphorylase 1.

Host

Rabbit

Positive Control

A431 cell lysate, mouse kidney tissue lysate, rat lung tissue lysate, rat kidney tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE56-62

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size: 34 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Uridine Phosphorylase 1

SYNONYMS

UDRPASE antibody; UP antibody; UPase 1 antibody; UPASE antibody; UPP antibody; Upp1 antibody; UPP1_HUMAN antibody; UrdPase 1 antibody; Uridine phosphorylase 1 antibody

SUBCELLULAR LOCATION

Cytosol, nucleoplasm.

FUNCTION

This gene encodes a uridine phosphorylase, an enzyme that catalyzes the reversible phosphorylation of uridine (or 2'- deoxyuridine) to uracil and ribose-1-phosphate (or deoxyribose-1-phosphate). The encoded enzyme functions in the degradation and salvage of pyrimidine ribonucleosides.