PRODUCT CODE: ER1803-08

UQCRC2 Rabbit Polyclonal Antibody (ER1803-08)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.
  • Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.
  • ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
  • ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
  • ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with UQCRC2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

UQCRC2 Rabbit Polyclonal Antibody (ER1803-08)

Immunogen

Recombinant protein within human uqcrc2 aa 90-370.

Host

Rabbit

Positive Control

Rat heart tissue, human liver tissue, mouse brain tissue, HepG2.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

UQCRC2

SYNONYMS

Complex III subunit 2 antibody; Core protein II antibody; Cytochrome b c1 complex subunit 2 mitochondrial antibody; Cytochrome b-c1 complex subunit 2 antibody; EC 1.10.2.2 antibody; MC3DN5 antibody; mitochondrial antibody; QCR2 antibody; QCR2_HUMAN antibody; Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody; Ubiquinol cytochrome c reductase core protein II antibody; Ubiquinol-cytochrome-c reductase complex core protein 2 antibody; UQCR2 antibody; Uqcrc2 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

Cytochrome c is a well characterized, mobile electron transport protein that is essential to energy conversion in all aerobic organisms. Cytochrome b associates with cytochrome c subunit 1 and the Rieske protein to form complex III (also designated cytochrome bc1 complex), which is involved in cellular respiration. Ubiquinol cytochrome c reductase (UQCRFS1), also referred to as Rieske iron-sulfur protein, represents an important subunit of complex III of the mitochondrial respiratory chain that transfers electrons from ubiquinol to cytochrome c. The UQCRFS1 complex is made up of 3 respiratory subunits (cytochrome b, cytochrome c1, Rieske protein), 2 core proteins, and 6 low-molecular weight proteins. Ubiquinol cytochrome-c reductase complex core protein 2 (UQCRC2) represents one of the core proteins of UQCRFS1, and it is required for the assembly of the complex.