UGP2 Mouse Monoclonal Antibody [7H1] (EM1701-76)

Western blot analysis of UGP2 on human liver tissue lysate using anti-UGP2 antibody at 1/200 dilution.

Applications

WB
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

UGP2 Mouse Monoclonal Antibody [7H1] (EM1701-76)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

Human liver tissue, rat skeletal muscle tissue, mouse heart tissue, LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7H1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG1

APPLICATION DILUTION

WB
1:500

IHC-P
1:50-1:100

FC
1:50-1:100

TARGET

UNIPROT #

Q16851

PROTEIN NAME

UGP2

SYNONYMS

pHC379 antibody; UDP glucose diphosphorylase antibody; UDP glucose pyrophosphorylase 1 antibody; UDP glucose pyrophosphorylase 2 antibody; UDP glucose pyrophosphorylase antibody; UDP-glucose pyrophosphorylase antibody; UDPG antibody; UDPGP 2 antibody; UDPGP antibody; UDPGP2 antibody; UGP 1 antibody; UGP 2 antibody; UGP1 antibody; Ugp2 antibody; UGPA_HUMAN antibody; UGPase 2 antibody; UGPase antibody; UGPP 1 antibody; UGPP 2 antibody; UGPP1 antibody; UGPP2 antibody; Uridyl diphosphate glucose pyrophosphorylase 1 antibody; Uridyl diphosphate glucose pyrophosphorylase 2 antibody; UTP glucose 1 phosphate uridyltransferase antibody; UTP glucose 1 phosphate uridylyltransferase 2 antibody; UTP glucose 1 phosphate uridylyltransferase antibody; UTP--glucose-1-phosphate uridylyltransferase antibody

SEQUENCE SIMILARITIES

Belongs to the UDPGP type 1 family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

UGP2 (UDP-glucose pyrophosphorylase 2), also known as UDPG, UGPP2, UDPGP2 or pHC379, is an evolutionarily conserved protein belonging to the UDPGP type 1 family of proteins. Localizing to the cytoplasm, UGP2 forms homooligomers and is believed to function as a glucosyl donor in cellular metabolic pathways. More specifically, UGP2 catalyzes the transfer of a glucose moiety from glucose-1-phosphate to UTP, producing a diphosphate and UDP-glucose. UDP-glucose is an essential precursor for the synthesis of polysaccharides; in liver and muscle, UDP-glucose is a precursor of glycogen, in liver UDP-glucose is also a precursor of UDP-glucuronate, and in lactating mammary gland UDP-glucose is converted to UDP-galactose and then to lactose.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY

The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)

Applications

REACTIVITY

Human

Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat