PRODUCT CODE: HA500025

UGGT1 Rabbit Polyclonal Antibody (HA500025)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of UGGT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat liver tissue lysate<br />
Lane 2: HL-60 cell lysate<br />
Lane 3: Mouse liver tissue lysate
  • Western blot analysis of UGGT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Rat liver tissue lysate<br />
Lane 2: HL-60 cell lysate<br />
Lane 3: Mouse liver tissue lysate
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-UGGT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500025, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of UGGT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500025, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat liver tissue lysate
Lane 2: HL-60 cell lysate
Lane 3: Mouse liver tissue lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

UGGT1 Rabbit Polyclonal Antibody (HA500025)

Immunogen

Recombinant protein within human uggt1 aa 1350-1555.

Host

Rabbit

Positive Control

Rat liver tissue lysate, HL-60 cell lysate, mouse liver tissue lysate, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

177 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

UGGT1

SYNONYMS

GT antibody; HUGT1 antibody; UDP glucose glycoprotein glucosyltransferase 1 antibody; UDP Glucose Glycoprotein Glucosyltransferase antibody; UDP--Glc:glycoprotein glucosyltransferase antibody; UDP-glucose ceramide glucosyltransferase-like 1 antibody; UDP-glucose:glycoprotein glucosyltransferase 1 antibody; UGCGL1 antibody; UGGG1 antibody; UGGG1_HUMAN antibody; UGGT antibody; Uggt1 antibody; UGT1 antibody; UGTR antibody

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

UGGT is part of the ER quality control system of glycoprotein folding and its activity increases the potential for correctly folded glycoproteins.The main proteins involved in the ER quality control system are UGGT, the ER lectin chaperones (calnexin and calreticulin), and glucosidase II. UGGT first recognizes the incompletely folded glycoprotein and monoglucosylates it. The lectins, calnexin and calreticulin, have high affinities for monoglucosylated proteins and the ER chaperones that associate with these lectins assist the folding of the misfolded glycoprotein. Subsequently, glucosidase II will deglucosylate the glycoprotein. If the glycoprotein is still misfolded, UGGT will re-glucosylate it and allow it to go through the cycle again.