PRODUCT CODE: ET1612-82

Ubiquitin-like modifier-activating enzyme 1 Recombinant Rabbit Monoclonal Antibody [SD08-62] (ET1612-82)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Ubiquitin-like modifier-activating enzyme 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse kidney tissue lysate<br />
Lane 2: mouse liver tissue lysate
  • Western blot analysis of Ubiquitin-like modifier-activating enzyme 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse kidney tissue lysate<br />
Lane 2: mouse liver tissue lysate
  • ICC staining of Ubiquitin-like modifier-activating enzyme 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Ubiquitin-like modifier-activating enzyme 1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Ubiquitin-like modifier-activating enzyme 1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-82, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Ubiquitin-like modifier-activating enzyme 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ubiquitin-like modifier-activating enzyme 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-82, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Ubiquitin-like modifier-activating enzyme 1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-82, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Ubiquitin-like modifier-activating enzyme 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse kidney tissue lysate
Lane 2: mouse liver tissue lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Ubiquitin-like modifier-activating enzyme 1 Recombinant Rabbit Monoclonal Antibody [SD08-62] (ET1612-82)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse kidney tissue lysate, mouse liver tissue lysate, Hela, SKOV-3, A549, mouse ovary tissue, human kidney tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-62

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

118 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin-like modifier-activating enzyme 1

SYNONYMS

A1S9 antibody; A1S9 protein antibody; A1S9T and BN75 temperature sensitivity complementing antibody; A1S9T antibody; A1ST antibody; AMCX1 antibody; CFAP124 antibody; CTD-2522E6.1 antibody; GXP 1 antibody; GXP1 antibody; MGC4781 antibody; POC20 antibody; POC20 centriolar protein homolog antibody; Protein A1S9 antibody; SMAX2 antibody; Uba1 antibody; UBA1, ubiquitin-activating enzyme E1 homolog A antibody; UBA1_HUMAN antibody; UBA1A antibody; UBE 1 antibody; UBE 1X antibody; UBE1 antibody; UBE1X antibody; Ubiquitin activating enzyme E1 antibody; Ubiquitin-activating enzyme E1 antibody; Ubiquitin-like modifier-activating enzyme 1 antibody

SEQUENCE SIMILARITIES

Belongs to the ubiquitin-activating E1 family.

TISSUE SPECIFICITY

Detected in erythrocytes (at protein level). Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

ISGylated.

SUBCELLULAR LOCATION

Nucleus, Mitochondrion, Cytoplasm.

FUNCTION

The ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. Specifically, UBE1 functions to adenylate the C-terminal glycine residue of ubiquitin, a reaction that is ATP-dependent and is proceeded by the formation of a thiolester bond with a cysteine residue of UBE1. The UBE1-activated ubiquitin is then transferred to a ubiquitin conjugated enzyme, which donates the ubiquitin residue to target substrates. The UBE1 gene is an example of an X-Y homologous gene, which is X-linked with a distinct Y-linked gene in many mammals. However, no UBE1 homolog is detectable on the human Y chromosome. UBE1 is thought to escape X inactivation in humans.