UBE4B Recombinant Rabbit Monoclonal Antibody [JE55-76] (ET7111-11)
Catalog# ET7111-11
Rabbit Monoclonal to UBE4B
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: Raji cell lysate (10 µg/Lane)
Lane 3: RAW264.7 cell lysate (10 µg/Lane)
Lane 4: PC-12 cell lysate (10 µg/Lane)
Lane 5: Mouse testis tissue lysate (20 µg/Lane)
Lane 6: Rat testis tissue lysate (20 µg/Lane)
Predicted band size: 146 kDa
Observed band size: 146 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-11) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
UBE4B Recombinant Rabbit Monoclonal Antibody [JE55-76] (ET7111-11)
Immunogen
Synthetic peptide within n-terminal human ube4b.
Host
Rabbit
Positive Control
HeLa cell lysate, Raji cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate, human lung carcinoma tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, rat skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE55-76
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 146 kDa.
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
UBE4B
SYNONYMS
4930551I19Rik antibody;4933406G05Rik antibody;AU014668 antibody;D4Bwg0973e antibody;E4 antibody;HDNB1 antibody;Homozygously deleted in neuroblastoma 1 antibody;KIAA0684 antibody;mKIAA0684 antibody;RP23 173D8.1 antibody;Ube4b antibody;UBE4B_HUMAN antibody;Ubiquitin conjugation factor E4 B antibody;Ubiquitin fusion degradation protein 2 antibody;UBOX3 antibody;UFD2 antibody;UFD2a antibody;Ufd2p antibody
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
CITATIONS
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Kong, X., Shu, X., Wang, J., Liu, D., Ni, Y., Zhao, W., Wang, L., Gao, Z., Chen, J., Yang, B., Guo, X., & Wang, Z.
Fine-tuning of mTOR signaling by the UBE4B-KLHL22 E3 ubiquitin ligase cascade in brain development
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WB
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Mouse
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Citation
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