Positive control:
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
UBA3 Mouse Monoclonal Antibody [1C10-5-5] (EM1901-65)
Immunogen
Recombinant protein within human uba3 aa 220-424 / 463.
Host
Mouse
Positive Control
HepG2 cell lysate, 293T cell lysate, human lung tissue, human lung carcinoma tissue, human skin tissue, human breast carcinoma tissue, human esophagus tissue, SiHa.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1C10-5-5
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
52 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
IHC-P:1:100-1:500
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
UBA3
SYNONYMS
DKFZp566J164 antibody; EC 6.3.2. antibody; hUba3 antibody; MGC22384 antibody; NEDD8 activating enzyme E1 catalytic subunit antibody; NEDD8 activating enzyme E1C antibody; Nedd8 activating enzyme hUba3 antibody; NEDD8-activating enzyme E1 catalytic subunit antibody; NEDD8-activating enzyme E1C antibody; uba3 antibody; UBA3 ubiquitin activating enzyme E1 homolog antibody; UBA3_HUMAN antibody; UBE1C antibody; Ubiquitin activating enzyme 3 antibody; Ubiquitin activating enzyme E1C antibody; Ubiquitin-activating enzyme 3 antibody; Ubiquitin-activating enzyme E1C antibody; Ubiquitin-like modifier-activating enzyme 3 antibody
SEQUENCE SIMILARITIES
Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
TISSUE SPECIFICITY
Ubiquitously expressed.
SUBCELLULAR LOCATION
Cytosol, nucleus, cytoplasm.
FUNCTION
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.