UAP1 Mouse Monoclonal Antibody [4H1] (EM1701-50)
Catalog# EM1701-50
Mouse Monoclonal to UAP1
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
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Human
-
Mouse
-
Rat
Western blot analysis of UAP1 on different lysates with Mouse anti-UAP1 antibody (EM1701-50) at 1/2,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 57/59 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-50) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-Br-3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 57/59 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-50) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.