PRODUCT CODE: ET7107-98

U1A Recombinant Rabbit Monoclonal Antibody [JB33-48] (ET7107-98)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Rat brain  <br />
  Lane 2: K562<br />
 Lane 3: A431  <br />
       Lane 4: Mouse kidney
  • Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Rat brain  <br />
  Lane 2: K562<br />
 Lane 3: A431  <br />
       Lane 4: Mouse kidney
  • ICC staining U1A in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining U1A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining U1A in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-U1A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-U1A antibody. Counter stained with hematoxylin.
Western blot analysis of U1A on different lysates using anti-U1A antibody at 1/500 dilution.
Positive control:
Lane 1: Rat brain
Lane 2: K562
Lane 3: A431
Lane 4: Mouse kidney

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

U1A Recombinant Rabbit Monoclonal Antibody [JB33-48] (ET7107-98)

Immunogen

Recombinant protein corresponding to n-terminal human u1a.

Host

Rabbit

Positive Control

Rat brain tissue lysate, K562, A431, mouse kidney tissue lysate, 293T, SH-SY-5Y, SiHa, human lung cancer tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB33-48

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

U1A

SYNONYMS

fc19d01 antibody; Mud1 antibody; small nuclear ribonucleoprotein polypeptide A antibody; snRNP A antibody; snRNP protein A antibody; SNRPA antibody; SNRPA_HUMAN antibody; U1 small nuclear ribonucleoprotein A antibody; U1 small nuclear RNP specific A antibody; U1 snRNP A antibody; U1 snRNP specific protein A antibody; U1-A antibody; U1A antibody; wu:fc19d01 antibody; zgc:77810 antibody

SEQUENCE SIMILARITIES

Belongs to the RRM U1 A/B'' family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

SNRPA (small nuclear ribonucleoprotein polypeptide A), also known as U1A (U1 snRNP protein A), is a component of the RNA spliceosome, a complex of proteins that are required for the precise excision of introns from pre-messenger RNA (pre-mRNA). Localizing to the nucleus, SNRPA contains two RRM (RNA recognition motif) domains, namely RRM1 and RRM2, and RRM1 specifically associates with the stem loop II of U1 snRNA (small nuclear RNA). In addition to functioning as a component of the U1 snRNP, SNRPA negatively regulates polyadenylation of SNRPA pre-mRNA, thereby negatively regulating itself. By inhibiting the addition of a polyA tail that would allow the pre-mRNA to mature, SNRPA causes the nuclear exosome degradation of the SNRPA pre-mRNA. At least 16% of cellular SNRPA also functions in an snRNP-free form (SF-A) that complexes with a group of non-snRNP proteins.