PRODUCT CODE: M1701-2

Tyrosinase Mouse Monoclonal Antibody [C2-B5] (M1701-2)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of tyrosinase on melanoma cells lysates using anti- tyrosinase antibody at 1/1,000 dilution.
  • Western blot analysis of tyrosinase on melanoma cells lysates using anti- tyrosinase antibody at 1/1,000 dilution.
  • ICC staining tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tyrosinase tissue using anti- tyrosinase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of B16F1 cells with tyrosinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody
Western blot analysis of tyrosinase on melanoma cells lysates using anti- tyrosinase antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Tyrosinase Mouse Monoclonal Antibody [C2-B5] (M1701-2)

Immunogen

Peptide linked to klh

Host

Mouse

Positive Control

Melanoma cells lysates, B16F1, human melanoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

C2-B5

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -391C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tyrosinase

SYNONYMS

ATN antibody; CMM8 antibody; LB24 AB antibody; LB24-AB antibody; Monophenol monooxygenase antibody; OCA1 antibody; OCA1A antibody; OCAIA antibody; Oculocutaneous albinism IA antibody; SHEP3 antibody; SK29 AB antibody; SK29-AB antibody; Tumor rejection antigen AB antibody; TYR antibody; TYRO_HUMAN antibody; tyrosinase (oculocutaneous albinism IA) antibody; Tyrosinase antibody

SEQUENCE SIMILARITIES

Belongs to the tyrosinase family.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone. Studies have shown tyrosinase to be a very specific marker for melanomas, not cross reacting with any other tumors or normal tissues tested Other studies have shown tyrosinase to be a more sensitive marker when compared to HMB-45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB-45. However, both tyrosinase and MART-1 negative staining was seen in those variants without an epidermal component. Unlike HMB-45, neither tyrosinase or MART-1 discriminates between activated or resting melanocytes. In conclusion, tyrosinase appears to be a superior melanoma marker when compared to HMB-45.