Tyk2 Rabbit Polyclonal Antibody (ER1803-05)
Catalog# ER1803-05
Rabbit Polyclonal to Tyk2
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Rat
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-05) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Tyk2 Rabbit Polyclonal Antibody (ER1803-05)
Immunogen
Recombinant protein within human tyk2 aa 390-620.
Host
Rabbit
Positive Control
Raji cell lysate, Jurkat cell lysate, A549 cell lysate, MCF-7, human liver carcinoma tissue, human colon tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 134 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Tyk2
SYNONYMS
JTK 1 antibody;JTK1 antibody;Non receptor tyrosine protein kinase 2 antibody;Non receptor tyrosine protein kinase TYK2 antibody;Non-receptor tyrosine-protein kinase TYK2 antibody;OTTHUMP00000232745 antibody;OTTHUMP00000232746 antibody;OTTHUMP00000232748 antibody;Protein Tyrosine Kinase 2 antibody;TYK 2 antibody;Tyk2 antibody;TYK2_HUMAN antibody;Tyrosine kinase 2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
TISSUE SPECIFICITY
Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
SUBCELLULAR LOCATION
Cytoskeleton. Nucleus. Cytosol. Plasma membrane.
FUNCTION
TYK2 belongs to the family of non-receptor janus tyrosine kinases, which regulate a spectrum of cellular functions occurring downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, recruit STAT transcription factors to the cytokine receptor where TYK2 is associated. TYK2 then phosphorylates proximal STAT factors, which subsequently dimerize, translocate to the nucleus, and bind to cis elements upstream of target gene promoters to regulating transcription. The canonical JAK-STAT pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Cytokine induced pro-inflammatory responses are attractive targets for anti-inflammatory therapies, specifically at the level of Jak-STAT signaling.
CITATIONS
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Du Xingde,et al
Environmentally related microcystin-LR-induced ovarian dysfunction via the CCL2-CCR10 axis in mice ameliorated by dietary mulberry
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WB
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Mouse
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Citation
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